Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 4-15
... phagemids Levinson et al . ( 1984 ) F ' traD36 proAB lacl lacZAM15 + r Rec Kan ' hsdR recA1 XS101 Used for growth of phagemids Levinson et al . ( 1984 ) F ' kan Will modify but not restrict transfected DNA recA strain supE Alac - proAB ...
... phagemids Levinson et al . ( 1984 ) F ' traD36 proAB lacl lacZAM15 + r Rec Kan ' hsdR recA1 XS101 Used for growth of phagemids Levinson et al . ( 1984 ) F ' kan Will modify but not restrict transfected DNA recA strain supE Alac - proAB ...
Page 4-17
... phagemids are described in Table 4.2 . • • • Phagemids have several attractive features : They provide the stability and high yields of double - stranded DNA that are characteristic of conventional plasmids . They circumvent the tedious ...
... phagemids are described in Table 4.2 . • • • Phagemids have several attractive features : They provide the stability and high yields of double - stranded DNA that are characteristic of conventional plasmids . They circumvent the tedious ...
Page 4-18
... phagemids that carry intergenic regions from which the segment responsible for interference has been deleted ( Cleary and Ray 1981 ; Zagursky and Berman 1984 ) or mutants of the helper that are resistant to interference ( see below ) ...
... phagemids that carry intergenic regions from which the segment responsible for interference has been deleted ( Cleary and Ray 1981 ; Zagursky and Berman 1984 ) or mutants of the helper that are resistant to interference ( see below ) ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml