Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 48
Page 1-27
... pipette tip attached to a vacuum line ( Figure 1.3 ) . Use a gentle vacuum and touch the tip to the surface disposable pipette tip pellet gentle suction vacuum traps vacuum line FIGURE 1.3 Aspiration of supernatants . Hold the open ...
... pipette tip attached to a vacuum line ( Figure 1.3 ) . Use a gentle vacuum and touch the tip to the surface disposable pipette tip pellet gentle suction vacuum traps vacuum line FIGURE 1.3 Aspiration of supernatants . Hold the open ...
Page 1-44
... pipette into the centrifuge tube containing CsCl prepared in step 2. The tip of the pipette should touch the bottom of the tube . Carefully load the clear , red solution ( from beneath the scum ) prepared in step 7 into the tube through ...
... pipette into the centrifuge tube containing CsCl prepared in step 2. The tip of the pipette should touch the bottom of the tube . Carefully load the clear , red solution ( from beneath the scum ) prepared in step 7 into the tube through ...
Page 7-20
... pipette , carefully remove the fluid above the level of the cushion ( upper mark on the outside of the tube ) . This part of the gradient contains the viscous cellular DNA , which is usually visible as a white band . With a fresh pipette ...
... pipette , carefully remove the fluid above the level of the cushion ( upper mark on the outside of the tube ) . This part of the gradient contains the viscous cellular DNA , which is usually visible as a white band . With a fresh pipette ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml