Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 70
Page 1-92
... plates . The colonies are consolidated on a master agar plate and on a nitrocellulose filter laid on the surface of a second agar plate . After a period of growth , the colonies are lysed in situ . The master plate is stored at 4 ° C ...
... plates . The colonies are consolidated on a master agar plate and on a nitrocellulose filter laid on the surface of a second agar plate . After a period of growth , the colonies are lysed in situ . The master plate is stored at 4 ° C ...
Page 2-65
... plate containing 30-35 ml of hardened bottom agar . A freshly poured plate equilibrated to room temperature gives the best results , but an older plate ( 1-4 days old ) gives a satisfactory yield . Adequate titers of most strains of ...
... plate containing 30-35 ml of hardened bottom agar . A freshly poured plate equilibrated to room temperature gives the best results , but an older plate ( 1-4 days old ) gives a satisfactory yield . Adequate titers of most strains of ...
Page 6-41
... plate , lay the plate on a pad of paper in a fume hood and pour a small quantity of siliconizing fluid ( e.g. , Sigmacote ) onto it . Wipe the fluid over the surface of the plate with a pad of Kimwipes , and then rinse the plate in ...
... plate , lay the plate on a pad of paper in a fume hood and pour a small quantity of siliconizing fluid ( e.g. , Sigmacote ) onto it . Wipe the fluid over the surface of the plate with a pad of Kimwipes , and then rinse the plate in ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml