Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 22
Page 2-114
... radioactive probes to nucleic acids immobilized on solid supports , see Chapter 9 , pages 9.47-9.51 . The following ... radioactive contamination of the water bath , the resealed bag containing radioactivity should be sealed inside a ...
... radioactive probes to nucleic acids immobilized on solid supports , see Chapter 9 , pages 9.47-9.51 . The following ... radioactive contamination of the water bath , the resealed bag containing radioactivity should be sealed inside a ...
Page 2-116
... radioactive ink . Radioactive ink is made by mixing a small amount of 32P with waterproof black drawing ink . We find it convenient to make the ink in three grades : very hot ( > 2000 cps on a hand - held minimonitor ) , hot ( > 500 cps ...
... radioactive ink . Radioactive ink is made by mixing a small amount of 32P with waterproof black drawing ink . We find it convenient to make the ink in three grades : very hot ( > 2000 cps on a hand - held minimonitor ) , hot ( > 500 cps ...
Page 6-21
... radioactive ink to align the film with the gel . Cover the labels with Scotch Tape . This prevents radioactive contamination of the film holder or intensifying screen . Autoradiograph the gel at room temperature or at -70 ° C with an ...
... radioactive ink to align the film with the gel . Cover the labels with Scotch Tape . This prevents radioactive contamination of the film holder or intensifying screen . Autoradiograph the gel at room temperature or at -70 ° C with an ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml