Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 53
Page 2-59
... recA HB101 гВ mВ supE supF hsdS DP50supF гв mв Rec supE supF hsdS recA ED8767 C strain rk ( P2 ) supE hsdR ( P2 ) rk ( P2 ) supF hsdR ( P2cox ) C - 1a Q359 NM539 λEam ɅDam ( N205 recA [ Aimm 434 cIts b2 red Eam Sam / ^ ] ) ( N205 recA ...
... recA HB101 гВ mВ supE supF hsdS DP50supF гв mв Rec supE supF hsdS recA ED8767 C strain rk ( P2 ) supE hsdR ( P2 ) rk ( P2 ) supF hsdR ( P2cox ) C - 1a Q359 NM539 λEam ɅDam ( N205 recA [ Aimm 434 cIts b2 red Eam Sam / ^ ] ) ( N205 recA ...
Page 2-96
... recA function in the lysogens can be tested as follows : i . Streak the strain being tested , a known recA ̄ strain , and a wild - type E. coli strain onto a plate as shown in Figure 2.7 ( top ) . ii . Use a piece of cardboard to cover ...
... recA function in the lysogens can be tested as follows : i . Streak the strain being tested , a known recA ̄ strain , and a wild - type E. coli strain onto a plate as shown in Figure 2.7 ( top ) . ii . Use a piece of cardboard to cover ...
Page 5-88
... RecA - coated single - stranded DNA Double - stranded target DNA FIGURE 5.5 Initial homologous pairing D - loop The use of RecA protein to produce a displacement loop in double - stranded DNA . Topoisomerase I ( Calf thymus ) ...
... RecA - coated single - stranded DNA Double - stranded target DNA FIGURE 5.5 Initial homologous pairing D - loop The use of RecA protein to produce a displacement loop in double - stranded DNA . Topoisomerase I ( Calf thymus ) ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml