Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 27
Page 2-13
... red , infected recA cells are phenotypically RecA , RecBC , and Red . The ... gam bacteriophages are plated on wild - type E. coli . Stahl and his co ... red ̄ gam bacteriophages can package the closed circular dimers produced by 0 - form ...
... red , infected recA cells are phenotypically RecA , RecBC , and Red . The ... gam bacteriophages are plated on wild - type E. coli . Stahl and his co ... red ̄ gam bacteriophages can package the closed circular dimers produced by 0 - form ...
Page 2-19
... red gam ̄ . + GENOTYPE Aam32 Bam1 lac5 bio256 KH54 srlλ4 ° nin5 QSR 80 GENETIC CHARACTERISTICS Amber mutations Suppressor required Deletions Substitutions Insertions red / gam status of vector red / gam status of recombinants chi site ...
... red gam ̄ . + GENOTYPE Aam32 Bam1 lac5 bio256 KH54 srlλ4 ° nin5 QSR 80 GENETIC CHARACTERISTICS Amber mutations Suppressor required Deletions Substitutions Insertions red / gam status of vector red / gam status of recombinants chi site ...
Page 2-35
... red gam region is lost in the recombinants , so Spi selection can be used in hosts lysogenic for bacteriophage P2 . ADASH and AFIX are identical , apart from their polycloning sites . These bac- teriophages have bacteriophage T7 and T3 ...
... red gam region is lost in the recombinants , so Spi selection can be used in hosts lysogenic for bacteriophage P2 . ADASH and AFIX are identical , apart from their polycloning sites . These bac- teriophages have bacteriophage T7 and T3 ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml