Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 63
Page 1-3
... Replication and Incompatibility Replication of plasmid DNA is carried out by subsets of enzymes used to duplicate the bacterial chromosome . However , different plasmids use differ- ent subsets and replicate to different extents in ...
... Replication and Incompatibility Replication of plasmid DNA is carried out by subsets of enzymes used to duplicate the bacterial chromosome . However , different plasmids use differ- ent subsets and replicate to different extents in ...
Page 3-19
... replication and two copies of cos oriented in tandem . This arrangement of cos sequences increases the efficiency ... replication , both derived from SV2 - gpt ( Mulligan and Berg 1980 ) . pCV107 contains the mouse dihydrofolate ...
... replication and two copies of cos oriented in tandem . This arrangement of cos sequences increases the efficiency ... replication , both derived from SV2 - gpt ( Mulligan and Berg 1980 ) . pCV107 contains the mouse dihydrofolate ...
Page 4-17
... REPLICATION DERIVED FROM A FILAMENTOUS BACTERIOPHAGE Several vectors have been developed that combine desirable features of both plasmids and filamentous bacteriophages . In their simplest form , these vectors are plasmids with a ColE1 ...
... REPLICATION DERIVED FROM A FILAMENTOUS BACTERIOPHAGE Several vectors have been developed that combine desirable features of both plasmids and filamentous bacteriophages . In their simplest form , these vectors are plasmids with a ColE1 ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml