Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 76
Page 2-92
... Restriction Enzymes Vectors such as the EMBL series , 2001 , ADASH , and Charon 34 , 35 , and 40 contain a series of restriction sites , arranged in opposite orientations , in polycloning sites at each end of the central stuffer ...
... Restriction Enzymes Vectors such as the EMBL series , 2001 , ADASH , and Charon 34 , 35 , and 40 contain a series of restriction sites , arranged in opposite orientations , in polycloning sites at each end of the central stuffer ...
Page 5-28
... enzyme . Because most manufacturers have optimized the reaction conditions for their particu- lar preparations , we recommend following the instructions on ... Enzymes Used in Molecular Cloning ~DIGESTING DNA WITH RESTRICTION ENZYMES 5 28.
... enzyme . Because most manufacturers have optimized the reaction conditions for their particu- lar preparations , we recommend following the instructions on ... Enzymes Used in Molecular Cloning ~DIGESTING DNA WITH RESTRICTION ENZYMES 5 28.
Page 5-40
... restriction enzymes . However , not all restriction enzymes work in buffers used for DNA polymerase reactions , and it is advisable to carry out pilot reactions with the particular batch of enzyme on hand . If the restriction enzyme ...
... restriction enzymes . However , not all restriction enzymes work in buffers used for DNA polymerase reactions , and it is advisable to carry out pilot reactions with the particular batch of enzyme on hand . If the restriction enzyme ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml