Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 73
Page 1-39
... room temperature . 7. Recover the nucleic acids by centrifugation at 5000 rpm for 15 minutes at room temperature in a Sorvall GS3 rotor ( or its equivalent ) . Salt may precipitate if centrifugation is carried out at 4 ° C . 8. Decant ...
... room temperature . 7. Recover the nucleic acids by centrifugation at 5000 rpm for 15 minutes at room temperature in a Sorvall GS3 rotor ( or its equivalent ) . Salt may precipitate if centrifugation is carried out at 4 ° C . 8. Decant ...
Page 1-40
... room temperature in a Sorvall SS34 rotor ( or its equivalent ) . 3. Decant the supernatant carefully , and invert the open tube to allow the last drops of supernatant to drain away . Rinse the pellet and the walls of the tube with 70 ...
... room temperature in a Sorvall SS34 rotor ( or its equivalent ) . 3. Decant the supernatant carefully , and invert the open tube to allow the last drops of supernatant to drain away . Rinse the pellet and the walls of the tube with 70 ...
Page 6-30
... temperature Agarose Gels A number of types of agarose are available in which hydroxyethyl groups have been ... room temperature , transfer it and its supporting glass plate to a horizontal surface in a cold room to ensure that the gel ...
... temperature Agarose Gels A number of types of agarose are available in which hydroxyethyl groups have been ... room temperature , transfer it and its supporting glass plate to a horizontal surface in a cold room to ensure that the gel ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml