Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 51
Page 2-86
... sample several times with 2 - butanol ( see Appendix E ) to reduce the volume to less than 3 ml . Alternatively , if the pooled sample is small , the DNA can be precipitated with ethanol without prior dialysis by diluting the sample ...
... sample several times with 2 - butanol ( see Appendix E ) to reduce the volume to less than 3 ml . Alternatively , if the pooled sample is small , the DNA can be precipitated with ethanol without prior dialysis by diluting the sample ...
Page 6-12
... samples , it is best to make the gel a little thicker or to concentrate the DNA by ethanol precipitation rather than to fill the slot completely . For most purposes , it is not necessary to use a fresh pipette tip for every sample as ...
... samples , it is best to make the gel a little thicker or to concentrate the DNA by ethanol precipitation rather than to fill the slot completely . For most purposes , it is not necessary to use a fresh pipette tip for every sample as ...
Page 7-54
... sample wells cut into the upper section of the manifold . Smooth away any air bubbles trapped between the upper ... samples on ice . Caution : Formaldehyde is toxic and should be handled with care in a chemical hood . Many batches of ...
... sample wells cut into the upper section of the manifold . Smooth away any air bubbles trapped between the upper ... samples on ice . Caution : Formaldehyde is toxic and should be handled with care in a chemical hood . Many batches of ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml