Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xxvii
... SCREENING EXPRESSION LIBRARIES CONSTRUCTED IN BACTERIOPHAGE A VECTORS 12.16 SCREENING BACTERIAL COLONIES 12.21 Preparation of Colonies for Screening 12.21 METHOD 1 12.21 METHOD 2 12.23 Processing Filters for Immunological Screening of ...
... SCREENING EXPRESSION LIBRARIES CONSTRUCTED IN BACTERIOPHAGE A VECTORS 12.16 SCREENING BACTERIAL COLONIES 12.21 Preparation of Colonies for Screening 12.21 METHOD 1 12.21 METHOD 2 12.23 Processing Filters for Immunological Screening of ...
Page xxxi
... Screening Bacteriophage M13 Plaques by Hybridization to Radiolabeled Oligonucleotides 15.68 Screening Bacterial Colonies by Hybridization to Radiolabeled Oligonucleotides 15.72 OLIGONUCLEOTIDE - DIRECTED MUTAGENESIS BY SELECTION AGAINST ...
... Screening Bacteriophage M13 Plaques by Hybridization to Radiolabeled Oligonucleotides 15.68 Screening Bacterial Colonies by Hybridization to Radiolabeled Oligonucleotides 15.72 OLIGONUCLEOTIDE - DIRECTED MUTAGENESIS BY SELECTION AGAINST ...
Page 2-43
... screening of either plaques or colonies . Recombinants are Lac because the insertion sites are in lacZ . The Sam100 mutation allows propagation and screening in suppressing hosts and intracellular ac- cumulation of the fusion protein in ...
... screening of either plaques or colonies . Recombinants are Lac because the insertion sites are in lacZ . The Sam100 mutation allows propagation and screening in suppressing hosts and intracellular ac- cumulation of the fusion protein in ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml