Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-13
... sequences . The final genetic factor to consider in choosing a vector is the presence of a chi site . Wild - type bacteriophage A contains no chi site , but mutants carrying an octanucleotide chi sequence were detected as suppressors of ...
... sequences . The final genetic factor to consider in choosing a vector is the presence of a chi site . Wild - type bacteriophage A contains no chi site , but mutants carrying an octanucleotide chi sequence were detected as suppressors of ...
Page 3-21
... sequences that enhance the expression of cloned genes . The bacteriophage T3 and T7 promoters form the basis of a simple method to prepare probes from the ends of large segments of DNA cloned in pWE15 and pWE16 . These probes may then ...
... sequences that enhance the expression of cloned genes . The bacteriophage T3 and T7 promoters form the basis of a simple method to prepare probes from the ends of large segments of DNA cloned in pWE15 and pWE16 . These probes may then ...
Page 5-53
... sequences , a large proportion of the sequences of the template will be copied by the enzyme , and if all of the primers are present at equal concentrations , all sequences of the template should be copied at equal frequencies . Note ...
... sequences , a large proportion of the sequences of the template will be copied by the enzyme , and if all of the primers are present at equal concentrations , all sequences of the template should be copied at equal frequencies . Note ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml