Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 4-44
... DNA is cloned by conventional techniques into plasmids pUC118 and / or pUC119 and propagated as double - stranded DNA . pUC118 and pUC119 are deriva- tives of pUC18 and pUC19 that contain a 476 - bp fragment derived from the intergenic ...
... DNA is cloned by conventional techniques into plasmids pUC118 and / or pUC119 and propagated as double - stranded DNA . pUC118 and pUC119 are deriva- tives of pUC18 and pUC19 that contain a 476 - bp fragment derived from the intergenic ...
Page 5-34
... DNA polymerase ) Taq DNA polymerase Reverse ( AmpliTaq TM ) transcriptase Reactions DNA polymerase 5 ' → 3 ' Exonuclease + + double - stranded DNA 5 ' → 3 ' single - stranded DNA 3 ' → 5 ' double - stranded DNA 3 ' → 5 ' + RNAase H ...
... DNA polymerase ) Taq DNA polymerase Reverse ( AmpliTaq TM ) transcriptase Reactions DNA polymerase 5 ' → 3 ' Exonuclease + + double - stranded DNA 5 ' → 3 ' single - stranded DNA 3 ' → 5 ' double - stranded DNA 3 ' → 5 ' + RNAase H ...
Page 7-66
... stranded DNA Probes Single - stranded DNA probes are prepared by one of two methods : · Separation of the strands of a fragment of double - stranded DNA • De novo synthesis of a strand from a single - stranded template Strand ...
... stranded DNA Probes Single - stranded DNA probes are prepared by one of two methods : · Separation of the strands of a fragment of double - stranded DNA • De novo synthesis of a strand from a single - stranded template Strand ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml