Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-42
... solution to 30 ° C to facilitate the dissolution of the salt . Mix the solution gently until the salt is dissolved . 2. Add 0.8 ml of a solution of ethidium bromide ( 10 mg / ml in water ) for every 10 ml of the DNA / CsCl solution ...
... solution to 30 ° C to facilitate the dissolution of the salt . Mix the solution gently until the salt is dissolved . 2. Add 0.8 ml of a solution of ethidium bromide ( 10 mg / ml in water ) for every 10 ml of the DNA / CsCl solution ...
Page 1-44
... solution to a Beckman Quick - Seal centrifuge tube ( or its equivalent ) . Put this aside until needed in step 8 . 3. If necessary , adjust the volume of the solution of plasmid DNA to exactly 3 ml with TE ( pH 8.0 ) . 4. Add 8.4 g of ...
... solution to a Beckman Quick - Seal centrifuge tube ( or its equivalent ) . Put this aside until needed in step 8 . 3. If necessary , adjust the volume of the solution of plasmid DNA to exactly 3 ml with TE ( pH 8.0 ) . 4. Add 8.4 g of ...
Page 1-78
... solution to 1 liter with pure H2O . Sterilize the solution by filtration through a disposable Nalgene filter . Dispense the solution into 40 - ml aliquots , and store the aliquots in tissue - culture flasks ( Corning 25100 or equivalent ) ...
... solution to 1 liter with pure H2O . Sterilize the solution by filtration through a disposable Nalgene filter . Dispense the solution into 40 - ml aliquots , and store the aliquots in tissue - culture flasks ( Corning 25100 or equivalent ) ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml