Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 4-24
... sterile tube ( 13 mm × 100 mm ) . 2. Touch the surface of the chosen plaque with the end of a sterile , 8 - cm - long wooden stick ( sometimes called applicator or orange stick ) . Immediately immerse the end of the stick in the LB ...
... sterile tube ( 13 mm × 100 mm ) . 2. Touch the surface of the chosen plaque with the end of a sterile , 8 - cm - long wooden stick ( sometimes called applicator or orange stick ) . Immediately immerse the end of the stick in the LB ...
Page 7-26
... sterile Dispocolumn ( Bio - Rad ) ( or a pasteur pipette , plugged with sterile glass wool that has been treated with diethyl pyrocarbonate [ DEPC ] and autoclaved [ see page 7.3 ] ) . Wash the column with 3 column volumes of sterile ...
... sterile Dispocolumn ( Bio - Rad ) ( or a pasteur pipette , plugged with sterile glass wool that has been treated with diethyl pyrocarbonate [ DEPC ] and autoclaved [ see page 7.3 ] ) . Wash the column with 3 column volumes of sterile ...
Page 7-35
... sterile water , and the solutions should then be treated overnight with 0.1 % diethyl pyrocarbonate ( DEPC ) at 37 ° C and heated to 100 ° C for 15 minutes . After the sucrose solutions have cooled to room temperature , sterile ...
... sterile water , and the solutions should then be treated overnight with 0.1 % diethyl pyrocarbonate ( DEPC ) at 37 ° C and heated to 100 ° C for 15 minutes . After the sucrose solutions have cooled to room temperature , sterile ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml