Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-86
... stock solution of X - gal ( 20 mg / ml in dimethylformamide ) and 4 μl of a solution of isopropylthio - B - D - galactoside ( IPTG ) ( 200 mg / ml ) . X - gal is very expensive . However , the cost can be minimized by spreading a ...
... stock solution of X - gal ( 20 mg / ml in dimethylformamide ) and 4 μl of a solution of isopropylthio - B - D - galactoside ( IPTG ) ( 200 mg / ml ) . X - gal is very expensive . However , the cost can be minimized by spreading a ...
Page 6-9
... solution . Take care - the agarose solution can become superheated and may boil violently if it has been heated for too long in the microwave oven . Undissolved agarose appears as ... Preparation of an Agarose Gel 6 9 STOCK SOLUTIONS B 9.
... solution . Take care - the agarose solution can become superheated and may boil violently if it has been heated for too long in the microwave oven . Undissolved agarose appears as ... Preparation of an Agarose Gel 6 9 STOCK SOLUTIONS B 9.
Page 6-39
... solution to 37 ° C to dissolve the chemicals . Caution : Acrylamide is a ... Stock solutions of acrylamide can easily be purified by stirring overnight ... solution is 7.0 or less , and store the solution in dark bottles at room ...
... solution to 37 ° C to dissolve the chemicals . Caution : Acrylamide is a ... Stock solutions of acrylamide can easily be purified by stirring overnight ... solution is 7.0 or less , and store the solution in dark bottles at room ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml