Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 6-54
... stored for several hours at room temperature . PMSF is usually stored as a 10 mM or 100 mM stock solution ( 1.74 or 17.4 mg / ml in isopropanol ) at -20 ° C . Blocks containing DNA can be stored for a few days in TE ( pH 7.6 ) at 4 ° C ...
... stored for several hours at room temperature . PMSF is usually stored as a 10 mM or 100 mM stock solution ( 1.74 or 17.4 mg / ml in isopropanol ) at -20 ° C . Blocks containing DNA can be stored for a few days in TE ( pH 7.6 ) at 4 ° C ...
Page 6-56
... stored for several hours at room temperature . PMSF is usually stored as a 10 mM or 100 mM stock solution ( 1.74 or 17.4 mg / ml in isopropanol ) at -20 ° C . Blocks containing DNA can be stored for a few days in TE ( pH 7.6 ) at 4 ° C ...
... stored for several hours at room temperature . PMSF is usually stored as a 10 mM or 100 mM stock solution ( 1.74 or 17.4 mg / ml in isopropanol ) at -20 ° C . Blocks containing DNA can be stored for a few days in TE ( pH 7.6 ) at 4 ° C ...
Page 7-18
... stored at -70 ° C . Cells grown in monolayers should be washed once with PBS , drained well , and either used immediately or quick - frozen in liquid nitrogen and stored at -70 ° C . This can be achieved as follows : 1. Make a shallow ...
... stored at -70 ° C . Cells grown in monolayers should be washed once with PBS , drained well , and either used immediately or quick - frozen in liquid nitrogen and stored at -70 ° C . This can be achieved as follows : 1. Make a shallow ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml