Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-55
... strains of E. coli that have been designed to serve as specific hosts . Table 2.2 provides descriptions of some of the genetic markers found in these strains . The strains themselves are listed in Table 2.3 and are more fully described ...
... strains of E. coli that have been designed to serve as specific hosts . Table 2.2 provides descriptions of some of the genetic markers found in these strains . The strains themselves are listed in Table 2.3 and are more fully described ...
Page 4-13
... strains carrying this mutation will be modified and therefore protected against restriction if the vector is sub- sequently introduced into an hsd strain of E. coli K. + • recA1 : The recA gene of E. coli codes for a DNA - dependent ...
... strains carrying this mutation will be modified and therefore protected against restriction if the vector is sub- sequently introduced into an hsd strain of E. coli K. + • recA1 : The recA gene of E. coli codes for a DNA - dependent ...
Page 5-15
... Strains of E. coli Most strains of E. coli contain two enzymes that methylate DNA - the dam methylase and the dcm methylase . dam METHYLASE This enzyme introduces methyl groups at the N ° position of adenine in the sequence 5 ′ GATC 3 ...
... Strains of E. coli Most strains of E. coli contain two enzymes that methylate DNA - the dam methylase and the dcm methylase . dam METHYLASE This enzyme introduces methyl groups at the N ° position of adenine in the sequence 5 ′ GATC 3 ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml