Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 57
Page 4-2
... strand • Site - directed mutagenesis using synthetic oligonucleotides Transcription terminator TRANSCRIPTION < P P > P > intergenic region ( + ) strand ori ( - ) strand P > P > P > VII IX VIII Transcription terminator IV Wild Type ( 6.4 ...
... strand • Site - directed mutagenesis using synthetic oligonucleotides Transcription terminator TRANSCRIPTION < P P > P > intergenic region ( + ) strand ori ( - ) strand P > P > P > VII IX VIII Transcription terminator IV Wild Type ( 6.4 ...
Page 4-3
... strand of the parental RF DNA ( Meyer et al . 1979 ) . E. coli DNA polymerase I then adds nucleotides to the free 3 ' - hydroxyl terminus , progressively displacing the original ( + ) strand from the circular ( - ) strand template ...
... strand of the parental RF DNA ( Meyer et al . 1979 ) . E. coli DNA polymerase I then adds nucleotides to the free 3 ' - hydroxyl terminus , progressively displacing the original ( + ) strand from the circular ( - ) strand template ...
Page 7-66
... strands of a fragment of double - stranded DNA • De novo synthesis of a strand from a single - stranded template Strand - separated probes are prepared by using restriction enzymes , singly or in combination , to generate a DNA fragment ...
... strands of a fragment of double - stranded DNA • De novo synthesis of a strand from a single - stranded template Strand - separated probes are prepared by using restriction enzymes , singly or in combination , to generate a DNA fragment ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml