Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xxii
... STRAND OF cDNA 8.11 SYNTHESIS OF THE SECOND STRAND OF cDNA 8.14 Self - priming 8.14 Replacement Synthesis of the Second Strand ... cDNA Cloning 8.53 9 CONSTRUCTING cDNA LIBRARIES IN BACTERIOPHAGE VECTORS 8.54 Precautions 8.54 xxii Contents.
... STRAND OF cDNA 8.11 SYNTHESIS OF THE SECOND STRAND OF cDNA 8.14 Self - priming 8.14 Replacement Synthesis of the Second Strand ... cDNA Cloning 8.53 9 CONSTRUCTING cDNA LIBRARIES IN BACTERIOPHAGE VECTORS 8.54 Precautions 8.54 xxii Contents.
Page 5-40
... strand of cDNA in cDNA cloning . 5. Synthesis of double - stranded DNA from single - stranded templates during in vitro mutagenesis . The Klenow fragment of E. coli DNA polymerase I can 5.40 Enzymes Used in Molecular Cloning Large ...
... strand of cDNA in cDNA cloning . 5. Synthesis of double - stranded DNA from single - stranded templates during in vitro mutagenesis . The Klenow fragment of E. coli DNA polymerase I can 5.40 Enzymes Used in Molecular Cloning Large ...
Page 5-53
... synthesis of the first strand of cDNA . Depending on the quality of the reverse transcriptase and the reaction conditions , the sequences at the 3 ' terminus of the template may be overrepresented in the cDNA . Oligonucleotides of random ...
... synthesis of the first strand of cDNA . Depending on the quality of the reverse transcriptase and the reaction conditions , the sequences at the 3 ' terminus of the template may be overrepresented in the cDNA . Oligonucleotides of random ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml