Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 52
Page 1-39
... supernatant as possible to a fresh bottle . Discard the viscous liquid remaining in the centrifuge bottle . The failure to form a compact pellet is usually a consequence of inadequate mixing of the bacterial lysate with Solution III ...
... supernatant as possible to a fresh bottle . Discard the viscous liquid remaining in the centrifuge bottle . The failure to form a compact pellet is usually a consequence of inadequate mixing of the bacterial lysate with Solution III ...
Page 1-40
... supernatant carefully , and invert the open tube to allow the last drops of supernatant to drain away . Rinse the pellet and the walls of the tube with 70 % ethanol at room temperature . Drain off the ethanol , and use a pasteur pipette ...
... supernatant carefully , and invert the open tube to allow the last drops of supernatant to drain away . Rinse the pellet and the walls of the tube with 70 % ethanol at room temperature . Drain off the ethanol , and use a pasteur pipette ...
Page 4-32
... supernatant to drain away from the precipitate . Use Kimwipes to remove the last traces of supernatant from the walls and neck of the bottle . • 4. Add 10 ml of a solution of 10 mM Tris Cl ( pH 8.0 ) to the bottle . Use a pasteur ...
... supernatant to drain away from the precipitate . Use Kimwipes to remove the last traces of supernatant from the walls and neck of the bottle . • 4. Add 10 ml of a solution of 10 mM Tris Cl ( pH 8.0 ) to the bottle . Use a pasteur ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml