Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 5-14
... sequences . In some cases , these tetranu- cleotides occur within hexanucleotide target sequences of other enzymes . For example , Mbol and Sau3AI recognize the sequence ↓ 5 ' ... GATC .. 3 ' . 3 ' CTAG . • 5 ' whereas BamHI recognizes ...
... sequences . In some cases , these tetranu- cleotides occur within hexanucleotide target sequences of other enzymes . For example , Mbol and Sau3AI recognize the sequence ↓ 5 ' ... GATC .. 3 ' . 3 ' CTAG . • 5 ' whereas BamHI recognizes ...
Page 5-15
... sequence 5 ′ GATC 3 ' ( Hattman et al . 1978 ) . The recognition sites of several restriction enzymes ( PvuI , BamHI ... target sequences only when methylated : mrr ( 6 - methyladenine [ mA ] ) , mcrA ( m5 CG [ m5C = 5 - methylcytosine ] ...
... sequence 5 ′ GATC 3 ' ( Hattman et al . 1978 ) . The recognition sites of several restriction enzymes ( PvuI , BamHI ... target sequences only when methylated : mrr ( 6 - methyladenine [ mA ] ) , mcrA ( m5 CG [ m5C = 5 - methylcytosine ] ...
Page 7-79
... target sequences in the mRNA preparation . If desired , the end - labeled cDNA can be extracted from the gel and sequenced by the Maxam - Gilbert method . It is also possible to sequence the cDNA directly by including ...
... target sequences in the mRNA preparation . If desired , the end - labeled cDNA can be extracted from the gel and sequenced by the Maxam - Gilbert method . It is also possible to sequence the cDNA directly by including ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml