Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-5
... transcription is initiated at two promoters , PL and PR ' which are located to the left and right , respectively , of the cI ( repressor ) gene ( see Figure 2.2 ) . The ... Transcription 2 5 DNA Replication 2 5 Late Transcription 2 5.
... transcription is initiated at two promoters , PL and PR ' which are located to the left and right , respectively , of the cI ( repressor ) gene ( see Figure 2.2 ) . The ... Transcription 2 5 DNA Replication 2 5 Late Transcription 2 5.
Page 2-8
... transcriptions are controlled by PR and PL , respectively ) activate leftward transcription from promoters PE and p , so that genes cI and int are expressed . The product of the cI gene represses early transcription and consequently ...
... transcriptions are controlled by PR and PL , respectively ) activate leftward transcription from promoters PE and p , so that genes cI and int are expressed . The product of the cI gene represses early transcription and consequently ...
Page 7-77
... transcribed from the opposite strand of the DNA template . These rare com- plementary transcripts are thought to be generated by transcription initiated by bacteriophage T7 and SP6 RNA polymerases at the ends of the template ( Schenborn ...
... transcribed from the opposite strand of the DNA template . These rare com- plementary transcripts are thought to be generated by transcription initiated by bacteriophage T7 and SP6 RNA polymerases at the ends of the template ( Schenborn ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml