Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xv
... VITRO 2.95 Maintenance and Testing of Lysogens of Bacteriophage 2.96 Preparation of Packaging Extracts and Packaging of Bacteriophage › DNA In Vitro 2.98 PROTOCOL I : PREPARATION OF PACKAGING EXTRACTS FROM TWO LYSOGENS 2.100 PROTOCOL I ...
... VITRO 2.95 Maintenance and Testing of Lysogens of Bacteriophage 2.96 Preparation of Packaging Extracts and Packaging of Bacteriophage › DNA In Vitro 2.98 PROTOCOL I : PREPARATION OF PACKAGING EXTRACTS FROM TWO LYSOGENS 2.100 PROTOCOL I ...
Page 2-124
... vitro packaging of coliphage lambda DNA . Gene 39 : 313 . 1987. Improved in vitro packag- ing of DNA . Methods Enzymol . 153 : 95 . Rosenberg , S.M. , M.M. Stahl , I. Koba- yashi , and F.W. Stahl . 1985. Improved in vitro packaging of ...
... vitro packaging of coliphage lambda DNA . Gene 39 : 313 . 1987. Improved in vitro packag- ing of DNA . Methods Enzymol . 153 : 95 . Rosenberg , S.M. , M.M. Stahl , I. Koba- yashi , and F.W. Stahl . 1985. Improved in vitro packaging of ...
Page 2-124
... vitro packaging of coliphage Sim , and A. Efstratiadis . 1978. The lambda DNA . Gene 39 : 313 . isolation of structural genes from li1987. Improved in vitro packagbraries of eucaryotic DNA . Cell 15 : 687 . ing of DNA . Methods Enzymol ...
... vitro packaging of coliphage Sim , and A. Efstratiadis . 1978. The lambda DNA . Gene 39 : 313 . isolation of structural genes from li1987. Improved in vitro packagbraries of eucaryotic DNA . Cell 15 : 687 . ing of DNA . Methods Enzymol ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml