New Methods for Culturing Cells from Nervous TissuesPhilippe Poindron, Pascale Piguet, Dr. Eckart Förster The last decades have seen the rise of neuroscience to one of the most dynamic biomedical disciplines. With the development of powerful new techniques it has been possible to investigate specific physiological and pathological neural mechanisms and thus to elucidate the etiology of many neurodegenerative and neuromuscular diseases. This first volume of the new book series 'BioValley Monographs' presents a whole range of methods developed to grow different kinds of neurons and glial cells in vitro. It is the result of a trinational cooperation between scientists of Neurex, the neuroscience network of the Upper Rhine valley. The articles are not just simple descriptions of bench techniques but discuss the unique features of each method, its difficulties, and tricks to avoid these pitfalls. The book covers cells growing in a dissociated state, organotypic slices which retain the architecture of neuronal networks in vivo, as well as cultures on architectured substrates allowing researchers to apply geometrical constraints in order to study the role and importance of synaptic connections more efficiently. Presenting expert guidelines to prepare, establish and maintain neural cell and tissue cultures, this book will be a valuable tool for all scientists and technicians interested in basic and applied research in neurobiology, neurology and neuropharmacology. No scientific library should be without it! |
Contents
A New Technique to Control the Architecture of Neuronal Networks | 23 |
Organotypic Cultures of the Rat Retina | 58 |
Cerebellar Slice Cultures | 74 |
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a-mannans activity adhesion antibody axons BDNF Biol BioValley bipolar cells Brain Res calcium imaging cell bodies cell culture cell layer cell types cellular cerebellar slice cultures cerebellum CNTF coculture cortex cortical neurons culture medium dendritic density dentate gyrus differentiation dish dissection electrodes embryos entorhinal excitatory explant cultures fibers fluorescence Förster functional GABAergic glass coverslips granule cells H₂O HBSS hippocampal neurons immunocytochemistry incubated inhibitory interneurons Invitrogen kinase lithography MAMI-96 cells mannose receptor mask membrane mg/ml mice microglial cells microscope mmol/l molecules morphology neocortex neural neurites neuron cultures neuronal networks neurons in culture Neurosci Neurosci Methods neurotrophic ng/ml organotypic slice cultures pattern photoreceptors photoresist plasma plating Poindron poly-lysine postnatal preparation protein protocol Purkinje cells rat retina retina retinal cell Rinse serum serum-free Sigma solution staining substrate survival synapses vitro vivo weeks in vitro Xaliproden µg/ml