Gene Cloning : An IntroductionAn introductory textbook updated to incorporate advances made since the first edition was published in 1986, but retaining its mission to serve undergraduates with no previous experience of the subject and experienced researchers new to gene cloning. Annotation copyrighted by Book News, Inc., Portland, OR |
From inside the book
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Page 109
... EcoRI ( c ) Shuttling a DNA fragment from PUC8 to M13mp8 Recombinant pUC8 О BamHI New DNA EcoRI BamHI M13mp8 о Restriction sites Restrict with BamHI and EcoRI Restrict EcoRI with BamHI and EcoRI Ligate BamHI New DNA EcoRI Recombinant ...
... EcoRI ( c ) Shuttling a DNA fragment from PUC8 to M13mp8 Recombinant pUC8 О BamHI New DNA EcoRI BamHI M13mp8 о Restriction sites Restrict with BamHI and EcoRI Restrict EcoRI with BamHI and EcoRI Ligate BamHI New DNA EcoRI Recombinant ...
Page 116
... EcoRI HindIII lacZ ' HindIII EcoRI M13mp8 M13mp9 ( c ) Shuttling DNA from M13mp8 to M13mp9 EcoRI HindIII J Recombinant M13mp8 HindIII HindIII Restrict Ligate EcoRI ( d ) DNA sequencing using M13mp8 and M13mp9 Recombinant M13mp8 2 DNA ...
... EcoRI HindIII lacZ ' HindIII EcoRI M13mp8 M13mp9 ( c ) Shuttling DNA from M13mp8 to M13mp9 EcoRI HindIII J Recombinant M13mp8 HindIII HindIII Restrict Ligate EcoRI ( d ) DNA sequencing using M13mp8 and M13mp9 Recombinant M13mp8 2 DNA ...
Page 120
Terence A. Brown. 5 EcoRI sites Infect E. coli cells producing EcoRI Normal DNA Very few plaques Plaque formed by mutant phage Only 3 EcoRI sites Repeat infection with mutant phage No EcoRI sites [ 8 ] Few more plaques Second mutant ...
Terence A. Brown. 5 EcoRI sites Infect E. coli cells producing EcoRI Normal DNA Very few plaques Plaque formed by mutant phage Only 3 EcoRI sites Repeat infection with mutant phage No EcoRI sites [ 8 ] Few more plaques Second mutant ...
Contents
Why gene cloning is important | 3 |
plasmids and bacteriophages | 12 |
Purification of DNA from living cells | 27 |
Copyright | |
12 other sections not shown
Common terms and phrases
amino acid antibody autoradiograph bacteriophage bacterium BamHI base pairing biotechnology carried cDNA chromosomal DNA cloned gene cloning vectors coded codons coli colonies complementary containing control sequence cosmid culture cystic fibrosis cystic fibrosis gene defective deletion digestion DNA fragment DNA sequencing double-stranded EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression gene Figure genomic library higher organisms host cell hybridization probing identified infection insulin introns labelled lacZ LEU2 ligated M13 vector medium membrane method molecular molecule Figure mRNA mutagenesis mutation normal nuclease nucleotide nucleotide sequence obtained oligonucleotide phage particles plant cells plaques plasmid polylinker polymerase polynucleotide produced promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication restriction endonuclease restriction fragment restriction sites result RFLP selectable marker signals single-stranded DNA specific sticky ends T-DNA techniques Ti plasmid transcription transformed translation product trpA types unique restriction vaccine virus viruses vitro vitro mutagenesis yeast