Gene Cloning : An IntroductionAn introductory textbook updated to incorporate advances made since the first edition was published in 1986, but retaining its mission to serve undergraduates with no previous experience of the subject and experienced researchers new to gene cloning. Annotation copyrighted by Book News, Inc., Portland, OR |
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Page 162
... cDNA strand ( Figure 8.7c ) , resulting in a double - stranded DNA fragment that can be ligated into a vector and cloned ( Figure 8.7d ) . The resulting cDNA clones will be representative of the mRNA present in the original preparation ...
... cDNA strand ( Figure 8.7c ) , resulting in a double - stranded DNA fragment that can be ligated into a vector and cloned ( Figure 8.7d ) . The resulting cDNA clones will be representative of the mRNA present in the original preparation ...
Page 168
... cDNA library is often prepared in order to obtain a clone of a gene expressed at a relatively high level in a particular cell type . In the example of a cDNA library from developing wheat seeds , a large proportion of the clones will be ...
... cDNA library is often prepared in order to obtain a clone of a gene expressed at a relatively high level in a particular cell type . In the example of a cDNA library from developing wheat seeds , a large proportion of the clones will be ...
Page 214
... cDNA clones prepared directly from the mRNA sample are available . For HRT the cDNA is denatured , immobilized on a nitrocellulose membrane , and incubated with the mRNA sample ( Figure 10.15 ) . The specific mRNA counterpart of the ...
... cDNA clones prepared directly from the mRNA sample are available . For HRT the cDNA is denatured , immobilized on a nitrocellulose membrane , and incubated with the mRNA sample ( Figure 10.15 ) . The specific mRNA counterpart of the ...
Contents
Why gene cloning is important | 3 |
plasmids and bacteriophages | 12 |
Purification of DNA from living cells | 27 |
Copyright | |
12 other sections not shown
Common terms and phrases
amino acid antibody autoradiograph bacteriophage bacterium BamHI base pairing biotechnology carried cDNA chromosomal DNA cloned gene cloning vectors coded codons coli colonies complementary containing control sequence cosmid culture cystic fibrosis cystic fibrosis gene defective deletion digestion DNA fragment DNA sequencing double-stranded EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression gene Figure genomic library higher organisms host cell hybridization probing identified infection insulin introns labelled lacZ LEU2 ligated M13 vector medium membrane method molecular molecule Figure mRNA mutagenesis mutation normal nuclease nucleotide nucleotide sequence obtained oligonucleotide phage particles plant cells plaques plasmid polylinker polymerase polynucleotide produced promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication restriction endonuclease restriction fragment restriction sites result RFLP selectable marker signals single-stranded DNA specific sticky ends T-DNA techniques Ti plasmid transcription transformed translation product trpA types unique restriction vaccine virus viruses vitro vitro mutagenesis yeast