Gene Cloning : An IntroductionAn introductory textbook updated to incorporate advances made since the first edition was published in 1986, but retaining its mission to serve undergraduates with no previous experience of the subject and experienced researchers new to gene cloning. Annotation copyrighted by Book News, Inc., Portland, OR |
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Page 94
... coded by bacterial gene B - galactosidase fragment coded by plasmid gene Complete B - galactosidase molecule ( b ) Screening for pUC8 recombinants Agar + X - gal + IPTG Blue colony non - recombinant White colony = recombinant Blue ...
... coded by bacterial gene B - galactosidase fragment coded by plasmid gene Complete B - galactosidase molecule ( b ) Screening for pUC8 recombinants Agar + X - gal + IPTG Blue colony non - recombinant White colony = recombinant Blue ...
Page 169
... coded by at least two codons each . Nevertheless , in most cases , the different codons for an individual amino acid are related . Alanine , for example , is coded by GCA , GCT , GCG and GCC , so two out of the three nucleotides of the ...
... coded by at least two codons each . Nevertheless , in most cases , the different codons for an individual amino acid are related . Alanine , for example , is coded by GCA , GCT , GCG and GCC , so two out of the three nucleotides of the ...
Page 214
... coded by a cloned gene . In many cases this analysis will not be necessary as the protein will have been characterized long before the gene cloning experiment is performed . On the other hand , there will be occasions when the ...
... coded by a cloned gene . In many cases this analysis will not be necessary as the protein will have been characterized long before the gene cloning experiment is performed . On the other hand , there will be occasions when the ...
Contents
Why gene cloning is important | 3 |
plasmids and bacteriophages | 12 |
Purification of DNA from living cells | 27 |
Copyright | |
12 other sections not shown
Common terms and phrases
amino acid antibody autoradiograph bacteriophage bacterium BamHI base pairing biotechnology carried cDNA chromosomal DNA cloned gene cloning vectors coded codons coli colonies complementary containing control sequence cosmid culture cystic fibrosis cystic fibrosis gene defective deletion digestion DNA fragment DNA sequencing double-stranded EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression gene Figure genomic library higher organisms host cell hybridization probing identified infection insulin introns labelled lacZ LEU2 ligated M13 vector medium membrane method molecular molecule Figure mRNA mutagenesis mutation normal nuclease nucleotide nucleotide sequence obtained oligonucleotide phage particles plant cells plaques plasmid polylinker polymerase polynucleotide produced promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication restriction endonuclease restriction fragment restriction sites result RFLP selectable marker signals single-stranded DNA specific sticky ends T-DNA techniques Ti plasmid transcription transformed translation product trpA types unique restriction vaccine virus viruses vitro vitro mutagenesis yeast