Gene Cloning : An IntroductionAn introductory textbook updated to incorporate advances made since the first edition was published in 1986, but retaining its mission to serve undergraduates with no previous experience of the subject and experienced researchers new to gene cloning. Annotation copyrighted by Book News, Inc., Portland, OR |
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Page 30
... density at 600 nm 1.0 0.5 1 0 0.4 0.8 1.2 1.6 2.0 Cell number ( x 109 ) per ml Detector Figure 3.2 Estimation of bacterial cell number by measurement of optical density . ( a ) A sample of the culture is placed in a glass cuvette and ...
... density at 600 nm 1.0 0.5 1 0 0.4 0.8 1.2 1.6 2.0 Cell number ( x 109 ) per ml Detector Figure 3.2 Estimation of bacterial cell number by measurement of optical density . ( a ) A sample of the culture is placed in a glass cuvette and ...
Page 39
... density ( g / cm3 ) Figure 3.10 CsCl density gradient centrifugation . ( a ) A CsCl density gradient produced by high - speed centrifugation . ( b ) Separation of protein , DNA and RNA in a density gradient . caesium and chloride ions ...
... density ( g / cm3 ) Figure 3.10 CsCl density gradient centrifugation . ( a ) A CsCl density gradient produced by high - speed centrifugation . ( b ) Separation of protein , DNA and RNA in a density gradient . caesium and chloride ions ...
Page 45
Terence A. Brown. ( a ) Culture density is too low λ inoculum О О О Bacteria Add phage All the cells are rapidly lysed low phage titre = ( b ) Culture density is too high 0 ( c ) Culture density is just right Culture is never completely ...
Terence A. Brown. ( a ) Culture density is too low λ inoculum О О О Bacteria Add phage All the cells are rapidly lysed low phage titre = ( b ) Culture density is too high 0 ( c ) Culture density is just right Culture is never completely ...
Contents
Why gene cloning is important | 3 |
plasmids and bacteriophages | 12 |
Purification of DNA from living cells | 27 |
Copyright | |
12 other sections not shown
Common terms and phrases
amino acid antibody autoradiograph bacteriophage bacterium BamHI base pairing biotechnology carried cDNA chromosomal DNA cloned gene cloning vectors coded codons coli colonies complementary containing control sequence cosmid culture cystic fibrosis cystic fibrosis gene defective deletion digestion DNA fragment DNA sequencing double-stranded EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression gene Figure genomic library higher organisms host cell hybridization probing identified infection insulin introns labelled lacZ LEU2 ligated M13 vector medium membrane method molecular molecule Figure mRNA mutagenesis mutation normal nuclease nucleotide nucleotide sequence obtained oligonucleotide phage particles plant cells plaques plasmid polylinker polymerase polynucleotide produced promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication restriction endonuclease restriction fragment restriction sites result RFLP selectable marker signals single-stranded DNA specific sticky ends T-DNA techniques Ti plasmid transcription transformed translation product trpA types unique restriction vaccine virus viruses vitro vitro mutagenesis yeast