Gene Cloning : An IntroductionAn introductory textbook updated to incorporate advances made since the first edition was published in 1986, but retaining its mission to serve undergraduates with no previous experience of the subject and experienced researchers new to gene cloning. Annotation copyrighted by Book News, Inc., Portland, OR |
From inside the book
Results 1-3 of 26
Page 66
... gel electrophoresis was developed . ( a ) Separation of molecules by gel electrophoresis DNA molecules , like proteins and many other biological compounds , carry an electric charge , negative in the case of DNA . Consequently , when ...
... gel electrophoresis was developed . ( a ) Separation of molecules by gel electrophoresis DNA molecules , like proteins and many other biological compounds , carry an electric charge , negative in the case of DNA . Consequently , when ...
Page 67
Terence A. Brown. ( a ) Standard electrophoresis DNA ( b ) Gel electrophoresis по Electrophorese Buffer DNA migrates towards the anode , but little separation into size classes occurs Buffer Gel DNA , loaded into a well cut out of the gel ...
Terence A. Brown. ( a ) Standard electrophoresis DNA ( b ) Gel electrophoresis по Electrophorese Buffer DNA migrates towards the anode , but little separation into size classes occurs Buffer Gel DNA , loaded into a well cut out of the gel ...
Page 182
... gel electrophoresis . In conventional gel electrophoresis , as described on p . 66 , the electric field is orientated along the length of the gel and the DNA molecules migrate in a straight line towards the positive pole ( Figure 9.3a ) ...
... gel electrophoresis . In conventional gel electrophoresis , as described on p . 66 , the electric field is orientated along the length of the gel and the DNA molecules migrate in a straight line towards the positive pole ( Figure 9.3a ) ...
Contents
Why gene cloning is important | 3 |
plasmids and bacteriophages | 12 |
Purification of DNA from living cells | 27 |
Copyright | |
12 other sections not shown
Common terms and phrases
amino acid antibody autoradiograph bacteriophage bacterium BamHI base pairing biotechnology carried cDNA chromosomal DNA cloned gene cloning vectors coded codons coli colonies complementary containing control sequence cosmid culture cystic fibrosis cystic fibrosis gene defective deletion digestion DNA fragment DNA sequencing double-stranded EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression gene Figure genomic library higher organisms host cell hybridization probing identified infection insulin introns labelled lacZ LEU2 ligated M13 vector medium membrane method molecular molecule Figure mRNA mutagenesis mutation normal nuclease nucleotide nucleotide sequence obtained oligonucleotide phage particles plant cells plaques plasmid polylinker polymerase polynucleotide produced promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication restriction endonuclease restriction fragment restriction sites result RFLP selectable marker signals single-stranded DNA specific sticky ends T-DNA techniques Ti plasmid transcription transformed translation product trpA types unique restriction vaccine virus viruses vitro vitro mutagenesis yeast