Gene Cloning : An IntroductionAn introductory textbook updated to incorporate advances made since the first edition was published in 1986, but retaining its mission to serve undergraduates with no previous experience of the subject and experienced researchers new to gene cloning. Annotation copyrighted by Book News, Inc., Portland, OR |
From inside the book
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Page 4
... GENE CLONING REQUIRES SPECIALIZED TOOLS AND TECHNIQUES 1.4.1 VEHICLES The central component of a gene cloning experiment is the vehicle , which transports the gene into the host cell and is responsible for its replication . To act as a ...
... GENE CLONING REQUIRES SPECIALIZED TOOLS AND TECHNIQUES 1.4.1 VEHICLES The central component of a gene cloning experiment is the vehicle , which transports the gene into the host cell and is responsible for its replication . To act as a ...
Page 6
... DNA molecules . These processes , and the ways they are utilized in gene cloning , are described in Chapter 5 . Table 1.1 Basic skills needed to carry out a simple gene cloning experiment 1. Preparation of pure samples of DNA 2. Cutting DNA ...
... DNA molecules . These processes , and the ways they are utilized in gene cloning , are described in Chapter 5 . Table 1.1 Basic skills needed to carry out a simple gene cloning experiment 1. Preparation of pure samples of DNA 2. Cutting DNA ...
Page 7
... cloning can provide a pure sample of an individual gene , separated from all the other genes that it normally shares the cell with . To understand exactly how this arises , consider a gene cloning experiment drawn in a slightly ...
... cloning can provide a pure sample of an individual gene , separated from all the other genes that it normally shares the cell with . To understand exactly how this arises , consider a gene cloning experiment drawn in a slightly ...
Contents
Why gene cloning is important | 3 |
plasmids and bacteriophages | 12 |
Purification of DNA from living cells | 27 |
Copyright | |
12 other sections not shown
Common terms and phrases
amino acid antibody autoradiograph bacteriophage bacterium BamHI base pairing biotechnology carried cDNA chromosomal DNA cloned gene cloning vectors coded codons coli colonies complementary containing control sequence cosmid culture cystic fibrosis cystic fibrosis gene defective deletion digestion DNA fragment DNA sequencing double-stranded EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression gene Figure genomic library higher organisms host cell hybridization probing identified infection insulin introns labelled lacZ LEU2 ligated M13 vector medium membrane method molecular molecule Figure mRNA mutagenesis mutation normal nuclease nucleotide nucleotide sequence obtained oligonucleotide phage particles plant cells plaques plasmid polylinker polymerase polynucleotide produced promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication restriction endonuclease restriction fragment restriction sites result RFLP selectable marker signals single-stranded DNA specific sticky ends T-DNA techniques Ti plasmid transcription transformed translation product trpA types unique restriction vaccine virus viruses vitro vitro mutagenesis yeast