Gene Cloning : An IntroductionAn introductory textbook updated to incorporate advances made since the first edition was published in 1986, but retaining its mission to serve undergraduates with no previous experience of the subject and experienced researchers new to gene cloning. Annotation copyrighted by Book News, Inc., Portland, OR |
From inside the book
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Page 50
... NUCLEASES Nucleases degrade DNA molecules by breaking the phosphodiester bonds that link one nucleotide to the next in a DNA strand . There are two distinct kinds of nuclease ( Figure 4.1 ) . 1. Exonucleases , which remove nucleotides ...
... NUCLEASES Nucleases degrade DNA molecules by breaking the phosphodiester bonds that link one nucleotide to the next in a DNA strand . There are two distinct kinds of nuclease ( Figure 4.1 ) . 1. Exonucleases , which remove nucleotides ...
Page 52
... nuclease III , which removes nucleotides only from the 3 ' - terminus ( see p . 78 for a description of the differences between the 3 ' - and 5 ' - termini of a polynucleotide ) . the other hand , the special group of enzymes called ...
... nuclease III , which removes nucleotides only from the 3 ' - terminus ( see p . 78 for a description of the differences between the 3 ' - and 5 ' - termini of a polynucleotide ) . the other hand , the special group of enzymes called ...
Page 55
... nuclease activities of DNA polymerase I are controlled by different parts of the enzyme molecule , and can be separated if the enzyme is cleaved in a particular way ( Figure 4.5c ) . The part of the enzyme that retains the polymerase ...
... nuclease activities of DNA polymerase I are controlled by different parts of the enzyme molecule , and can be separated if the enzyme is cleaved in a particular way ( Figure 4.5c ) . The part of the enzyme that retains the polymerase ...
Contents
Why gene cloning is important | 3 |
plasmids and bacteriophages | 12 |
Purification of DNA from living cells | 27 |
Copyright | |
12 other sections not shown
Common terms and phrases
amino acid antibody autoradiograph bacteriophage bacterium BamHI base pairing biotechnology carried cDNA chromosomal DNA cloned gene cloning vectors coded codons coli colonies complementary containing control sequence cosmid culture cystic fibrosis cystic fibrosis gene defective deletion digestion DNA fragment DNA sequencing double-stranded EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression gene Figure genomic library higher organisms host cell hybridization probing identified infection insulin introns labelled lacZ LEU2 ligated M13 vector medium membrane method molecular molecule Figure mRNA mutagenesis mutation normal nuclease nucleotide nucleotide sequence obtained oligonucleotide phage particles plant cells plaques plasmid polylinker polymerase polynucleotide produced promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication restriction endonuclease restriction fragment restriction sites result RFLP selectable marker signals single-stranded DNA specific sticky ends T-DNA techniques Ti plasmid transcription transformed translation product trpA types unique restriction vaccine virus viruses vitro vitro mutagenesis yeast