Gene Cloning : An IntroductionAn introductory textbook updated to incorporate advances made since the first edition was published in 1986, but retaining its mission to serve undergraduates with no previous experience of the subject and experienced researchers new to gene cloning. Annotation copyrighted by Book News, Inc., Portland, OR |
From inside the book
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Page 111
... polylinker , that consists of a series of restriction sites and has EcoRI sticky ends ( Figure 6.7a ) . This polylinker was inserted into the EcoRI site of M13mp2 , to give M13mp7 , a more complex vector with four possible cloning sites ...
... polylinker , that consists of a series of restriction sites and has EcoRI sticky ends ( Figure 6.7a ) . This polylinker was inserted into the EcoRI site of M13mp2 , to give M13mp7 , a more complex vector with four possible cloning sites ...
Page 113
... Polylinker M13mp7 Figure 6.7 Construction of M13mp7 . ( a ) The polylinker and ( b ) its insertion into the EcoRl site of M13mp2 . plasmid pUC8 ( p . 108 ) . As with the plasmid vector , one advantage of M13mp8 is its ability to take ...
... Polylinker M13mp7 Figure 6.7 Construction of M13mp7 . ( a ) The polylinker and ( b ) its insertion into the EcoRl site of M13mp2 . plasmid pUC8 ( p . 108 ) . As with the plasmid vector , one advantage of M13mp8 is its ability to take ...
Page 114
... polylinker Sticky ends ( b ) Religation with new DNA- possible products ( c ) Colour of plaques on X - gal agar 1 New DNA inserts : New DNA 2 Polylinker reinserts : Polylinker 3 Nothing inserts - self - ligation : lacZ ' disrupted No B ...
... polylinker Sticky ends ( b ) Religation with new DNA- possible products ( c ) Colour of plaques on X - gal agar 1 New DNA inserts : New DNA 2 Polylinker reinserts : Polylinker 3 Nothing inserts - self - ligation : lacZ ' disrupted No B ...
Contents
Why gene cloning is important | 3 |
plasmids and bacteriophages | 12 |
Purification of DNA from living cells | 27 |
Copyright | |
12 other sections not shown
Common terms and phrases
amino acid antibody autoradiograph bacteriophage bacterium BamHI base pairing biotechnology carried cDNA chromosomal DNA cloned gene cloning vectors coded codons coli colonies complementary containing control sequence cosmid culture cystic fibrosis cystic fibrosis gene defective deletion digestion DNA fragment DNA sequencing double-stranded EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression gene Figure genomic library higher organisms host cell hybridization probing identified infection insulin introns labelled lacZ LEU2 ligated M13 vector medium membrane method molecular molecule Figure mRNA mutagenesis mutation normal nuclease nucleotide nucleotide sequence obtained oligonucleotide phage particles plant cells plaques plasmid polylinker polymerase polynucleotide produced promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication restriction endonuclease restriction fragment restriction sites result RFLP selectable marker signals single-stranded DNA specific sticky ends T-DNA techniques Ti plasmid transcription transformed translation product trpA types unique restriction vaccine virus viruses vitro vitro mutagenesis yeast