Gene Cloning : An IntroductionAn introductory textbook updated to incorporate advances made since the first edition was published in 1986, but retaining its mission to serve undergraduates with no previous experience of the subject and experienced researchers new to gene cloning. Annotation copyrighted by Book News, Inc., Portland, OR |
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Page 230
... promoter and will therefore be transcribed in the bacterium . 11.2.2 THE PROMOTER MUST BE CHOSEN WITH CARE The two short sequences shown in Figure 11.3a are consensus sequences , averages of all the E. coli promoter sequences that are ...
... promoter and will therefore be transcribed in the bacterium . 11.2.2 THE PROMOTER MUST BE CHOSEN WITH CARE The two short sequences shown in Figure 11.3a are consensus sequences , averages of all the E. coli promoter sequences that are ...
Page 231
Terence A. Brown. ( a ) A strong promoter Gene Transcription Strong promoter ( b ) A weak promoter Gene Transcription Weak Numerous transcripts Translation зв promoter Relatively few transcripts Translation لله صه B 8 る В Numerous ...
Terence A. Brown. ( a ) A strong promoter Gene Transcription Strong promoter ( b ) A weak promoter Gene Transcription Weak Numerous transcripts Translation зв promoter Relatively few transcripts Translation لله صه B 8 る В Numerous ...
Page 233
... promoter -35 -10 ( d ) XP , promoter -35 -10 IPTG Transcription < 30 ° C No transcription > 30 ° C Transcription Figure 11.8 Four promoters often used in expression vectors . The lac and trp promoters are shown upstream for the genes ...
... promoter -35 -10 ( d ) XP , promoter -35 -10 IPTG Transcription < 30 ° C No transcription > 30 ° C Transcription Figure 11.8 Four promoters often used in expression vectors . The lac and trp promoters are shown upstream for the genes ...
Contents
Why gene cloning is important | 3 |
plasmids and bacteriophages | 12 |
Purification of DNA from living cells | 27 |
Copyright | |
12 other sections not shown
Common terms and phrases
amino acid antibody autoradiograph bacteriophage bacterium BamHI base pairing biotechnology carried cDNA chromosomal DNA cloned gene cloning vectors coded codons coli colonies complementary containing control sequence cosmid culture cystic fibrosis cystic fibrosis gene defective deletion digestion DNA fragment DNA sequencing double-stranded EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression gene Figure genomic library higher organisms host cell hybridization probing identified infection insulin introns labelled lacZ LEU2 ligated M13 vector medium membrane method molecular molecule Figure mRNA mutagenesis mutation normal nuclease nucleotide nucleotide sequence obtained oligonucleotide phage particles plant cells plaques plasmid polylinker polymerase polynucleotide produced promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication restriction endonuclease restriction fragment restriction sites result RFLP selectable marker signals single-stranded DNA specific sticky ends T-DNA techniques Ti plasmid transcription transformed translation product trpA types unique restriction vaccine virus viruses vitro vitro mutagenesis yeast