Gene Cloning : An IntroductionAn introductory textbook updated to incorporate advances made since the first edition was published in 1986, but retaining its mission to serve undergraduates with no previous experience of the subject and experienced researchers new to gene cloning. Annotation copyrighted by Book News, Inc., Portland, OR |
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Page 118
... region of the λ DNA molecule can be removed without affecting the ability of the phage to infect E. coli cells . Removal of all , or part , of this non - essential region , between positions 20 and 35 on the map shown in Figure 2.9 ...
... region of the λ DNA molecule can be removed without affecting the ability of the phage to infect E. coli cells . Removal of all , or part , of this non - essential region , between positions 20 and 35 on the map shown in Figure 2.9 ...
Page 197
... region of a DNA molecule ( Figure 9.14 ) . Any region of any DNA molecule can be chosen , so long as the sequences at the borders of the region are known . This is because in order to carry out DNA amplification by PCR , two short ...
... region of a DNA molecule ( Figure 9.14 ) . Any region of any DNA molecule can be chosen , so long as the sequences at the borders of the region are known . This is because in order to carry out DNA amplification by PCR , two short ...
Page 207
... region of DNA that can bind a regulatory protein . It should therefore be possible to identify control sequences upstream of a cloned gene by searching the relevant region for protein - binding sites . There are two different approaches ...
... region of DNA that can bind a regulatory protein . It should therefore be possible to identify control sequences upstream of a cloned gene by searching the relevant region for protein - binding sites . There are two different approaches ...
Contents
Why gene cloning is important | 3 |
plasmids and bacteriophages | 12 |
Purification of DNA from living cells | 27 |
Copyright | |
12 other sections not shown
Common terms and phrases
amino acid antibody autoradiograph bacteriophage bacterium BamHI base pairing biotechnology carried cDNA chromosomal DNA cloned gene cloning vectors coded codons coli colonies complementary containing control sequence cosmid culture cystic fibrosis cystic fibrosis gene defective deletion digestion DNA fragment DNA sequencing double-stranded EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression gene Figure genomic library higher organisms host cell hybridization probing identified infection insulin introns labelled lacZ LEU2 ligated M13 vector medium membrane method molecular molecule Figure mRNA mutagenesis mutation normal nuclease nucleotide nucleotide sequence obtained oligonucleotide phage particles plant cells plaques plasmid polylinker polymerase polynucleotide produced promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication restriction endonuclease restriction fragment restriction sites result RFLP selectable marker signals single-stranded DNA specific sticky ends T-DNA techniques Ti plasmid transcription transformed translation product trpA types unique restriction vaccine virus viruses vitro vitro mutagenesis yeast