Gene Cloning : An IntroductionAn introductory textbook updated to incorporate advances made since the first edition was published in 1986, but retaining its mission to serve undergraduates with no previous experience of the subject and experienced researchers new to gene cloning. Annotation copyrighted by Book News, Inc., Portland, OR |
From inside the book
Results 1-3 of 55
Page 110
... replication , and most , if not all , of the enzymes needed for replication are provided by the host cell . Elaborate manipulations , such as those that resulted in pBR322 ( Figure 6.2a ) , are therefore possible so long as the final ...
... replication , and most , if not all , of the enzymes needed for replication are provided by the host cell . Elaborate manipulations , such as those that resulted in pBR322 ( Figure 6.2a ) , are therefore possible so long as the final ...
Page 133
... replication , which are the positions along the chromosome at which DNA replication initiates , similar to the origin of replication of a plasmid ( p . 12 ) . Once chromosome structure had been defined in this way the possibility arose ...
... replication , which are the positions along the chromosome at which DNA replication initiates , similar to the origin of replication of a plasmid ( p . 12 ) . Once chromosome structure had been defined in this way the possibility arose ...
Page 276
... replication in a host organism , into which a gene is inserted to construct a recombinant DNA molecule . Vehicle ... replication . Yeast integrative plasmid ( YIp ) A yeast vector that relies on integration into the host chromosome for ...
... replication in a host organism , into which a gene is inserted to construct a recombinant DNA molecule . Vehicle ... replication . Yeast integrative plasmid ( YIp ) A yeast vector that relies on integration into the host chromosome for ...
Contents
Why gene cloning is important | 3 |
plasmids and bacteriophages | 12 |
Purification of DNA from living cells | 27 |
Copyright | |
12 other sections not shown
Common terms and phrases
amino acid antibody autoradiograph bacteriophage bacterium BamHI base pairing biotechnology carried cDNA chromosomal DNA cloned gene cloning vectors coded codons coli colonies complementary containing control sequence cosmid culture cystic fibrosis cystic fibrosis gene defective deletion digestion DNA fragment DNA sequencing double-stranded EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression gene Figure genomic library higher organisms host cell hybridization probing identified infection insulin introns labelled lacZ LEU2 ligated M13 vector medium membrane method molecular molecule Figure mRNA mutagenesis mutation normal nuclease nucleotide nucleotide sequence obtained oligonucleotide phage particles plant cells plaques plasmid polylinker polymerase polynucleotide produced promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication restriction endonuclease restriction fragment restriction sites result RFLP selectable marker signals single-stranded DNA specific sticky ends T-DNA techniques Ti plasmid transcription transformed translation product trpA types unique restriction vaccine virus viruses vitro vitro mutagenesis yeast