Gene Cloning : An IntroductionAn introductory textbook updated to incorporate advances made since the first edition was published in 1986, but retaining its mission to serve undergraduates with no previous experience of the subject and experienced researchers new to gene cloning. Annotation copyrighted by Book News, Inc., Portland, OR |
From inside the book
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Page 24
... DNA molecule ) creates the single - stranded sticky ends , and also acts in conjunc- tion with other proteins to package the λ genomes into the phage head structure ... single - stranded DNA into 24 / Vehicles : plasmids and bacteriophages.
... DNA molecule ) creates the single - stranded sticky ends , and also acts in conjunc- tion with other proteins to package the λ genomes into the phage head structure ... single - stranded DNA into 24 / Vehicles : plasmids and bacteriophages.
Page 25
... stranded molecules ( c ) Mature M13 phage are continuously produced M13 particle injects DNA Pilus into cell O Single- stranded DNA Double - stranded DNA - replicative form ( RF ) RF replicates by rolling circle mechanism to produce ...
... stranded molecules ( c ) Mature M13 phage are continuously produced M13 particle injects DNA Pilus into cell O Single- stranded DNA Double - stranded DNA - replicative form ( RF ) RF replicates by rolling circle mechanism to produce ...
Page 54
Terence A. Brown. 4.1.2 LIGASES In the cell the function of DNA ligase is to repair single - stranded breaks ( ' discontinuities ' ) that arise in double - stranded DNA molecules during , for example , DNA replication . DNA ligases from ...
Terence A. Brown. 4.1.2 LIGASES In the cell the function of DNA ligase is to repair single - stranded breaks ( ' discontinuities ' ) that arise in double - stranded DNA molecules during , for example , DNA replication . DNA ligases from ...
Contents
Why gene cloning is important | 3 |
plasmids and bacteriophages | 12 |
Purification of DNA from living cells | 27 |
Copyright | |
12 other sections not shown
Common terms and phrases
amino acid antibody autoradiograph bacteriophage bacterium BamHI base pairing biotechnology carried cDNA chromosomal DNA cloned gene cloning vectors coded codons coli colonies complementary containing control sequence cosmid culture cystic fibrosis cystic fibrosis gene defective deletion digestion DNA fragment DNA sequencing double-stranded EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression gene Figure genomic library higher organisms host cell hybridization probing identified infection insulin introns labelled lacZ LEU2 ligated M13 vector medium membrane method molecular molecule Figure mRNA mutagenesis mutation normal nuclease nucleotide nucleotide sequence obtained oligonucleotide phage particles plant cells plaques plasmid polylinker polymerase polynucleotide produced promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication restriction endonuclease restriction fragment restriction sites result RFLP selectable marker signals single-stranded DNA specific sticky ends T-DNA techniques Ti plasmid transcription transformed translation product trpA types unique restriction vaccine virus viruses vitro vitro mutagenesis yeast