In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 152
... addition , what are the disadvantages to using primary cell cultures ? Finally , how do the data obtained from toxicolog- ical studies derived from primary myocardial cell cultures compare to data derived from whole - animal studies and ...
... addition , what are the disadvantages to using primary cell cultures ? Finally , how do the data obtained from toxicolog- ical studies derived from primary myocardial cell cultures compare to data derived from whole - animal studies and ...
Page 179
... addition ( 0.5-5 mM ; normal range 1.6-2.4 mM ) results in a sustained increase in developed force attributed exclu- sively to calcium entry independent of any contribution from calcium release . Specific receptor - mediated calcium ...
... addition ( 0.5-5 mM ; normal range 1.6-2.4 mM ) results in a sustained increase in developed force attributed exclu- sively to calcium entry independent of any contribution from calcium release . Specific receptor - mediated calcium ...
Page 406
... addition of pyruvate and cells , first in the ab- sence and then in the presence of 0.1 % ( v / v ) Triton X - 100 . The ratio of the slopes in the two cases gives the fraction of damaged cells . After purification , greater than 90 ...
... addition of pyruvate and cells , first in the ab- sence and then in the presence of 0.1 % ( v / v ) Triton X - 100 . The ratio of the slopes in the two cases gives the fraction of damaged cells . After purification , greater than 90 ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml