In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 199
... appears to occur by a different trans- port system than that on the basolateral side and is the first step in the ... appear to release GSH during isola- tion and are relatively deficient in GSH as prepared and if incubated in the ab ...
... appears to occur by a different trans- port system than that on the basolateral side and is the first step in the ... appear to release GSH during isola- tion and are relatively deficient in GSH as prepared and if incubated in the ab ...
Page 209
... appear dilated . Serum - Free Medium After 1 day in culture the normal architecture of the mucosa is retained in 70-80 % of the explants ( Fig . 6 ) . A similar picture is evident after 3 days in cul- ture ( Fig . 7 ) . Although there ...
... appear dilated . Serum - Free Medium After 1 day in culture the normal architecture of the mucosa is retained in 70-80 % of the explants ( Fig . 6 ) . A similar picture is evident after 3 days in cul- ture ( Fig . 7 ) . Although there ...
Page 345
... appear to restrict lu- minal accessibility , as Rodeheaver and co - workers ( 15 ) reported comparable luminal patencies for enzymatically derived tubules which exhibit shape changes and mechanically isolated segments which retain ...
... appear to restrict lu- minal accessibility , as Rodeheaver and co - workers ( 15 ) reported comparable luminal patencies for enzymatically derived tubules which exhibit shape changes and mechanically isolated segments which retain ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml