In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page xix
... approach is employed as a model for inves- tigative research or as a toxicity test for risk assessment , a critical component is the biological system . Successful preparation and maintenance of the appropri- ate cell , tissue , or ...
... approach is employed as a model for inves- tigative research or as a toxicity test for risk assessment , a critical component is the biological system . Successful preparation and maintenance of the appropri- ate cell , tissue , or ...
Page 280
... approach which will ensure that metabolism of the agent has occurred . On the negative side , this approach possesses many of the inherent limitations which are normally associated with whole - animal studies . The most serious are ...
... approach which will ensure that metabolism of the agent has occurred . On the negative side , this approach possesses many of the inherent limitations which are normally associated with whole - animal studies . The most serious are ...
Page 374
... approach is the ability to manipulate components of the medium to determine the role of selected factors in the biological response of interest . Such strategies have led to the discovery of novel growth substances and in- hibitors ...
... approach is the ability to manipulate components of the medium to determine the role of selected factors in the biological response of interest . Such strategies have led to the discovery of novel growth substances and in- hibitors ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml