In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 18
... attached DRG away from the vertebral column . This process is repeated in the caudal direction until the entire cord with attached DRG is free . The isolated cord is then crosscut between DRG into transverse sections about 0.5 mm thick ...
... attached DRG away from the vertebral column . This process is repeated in the caudal direction until the entire cord with attached DRG is free . The isolated cord is then crosscut between DRG into transverse sections about 0.5 mm thick ...
Page 143
... attached cells at different time points ) . The main Fig . 5A shows the cumulative number of cells produced in the ... Attached plus cumulative exfoliated cells ; inset shows attached cells only . ( B ) DNA synthesis ; asterisks indicate ...
... attached cells at different time points ) . The main Fig . 5A shows the cumulative number of cells produced in the ... Attached plus cumulative exfoliated cells ; inset shows attached cells only . ( B ) DNA synthesis ; asterisks indicate ...
Page 273
... attached , that is , 4 to 12 hr later , new medium with or without fetal calf serum and with 3 μM hydrocortisone hemisuccinate is added . Later on the medium is refreshed daily . About 60 % of hepatocytes attach to plastic ; the ...
... attached , that is , 4 to 12 hr later , new medium with or without fetal calf serum and with 3 μM hydrocortisone hemisuccinate is added . Later on the medium is refreshed daily . About 60 % of hepatocytes attach to plastic ; the ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml