In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 20
... axons projecting downward from the opposite pole . Holmes silver stain , × 358 . [ Reporduced with permission from F. J. Seil , Brain Res . 42 , 33 ( 1972 ) . ] ( C ) Purkinje cell axons converging from above on their target deep ...
... axons projecting downward from the opposite pole . Holmes silver stain , × 358 . [ Reporduced with permission from F. J. Seil , Brain Res . 42 , 33 ( 1972 ) . ] ( C ) Purkinje cell axons converging from above on their target deep ...
Page 23
... axons project away from cortex , are illustrated in Fig . 1B . The axons of Purkinje cells converge on the deep nucleus neurons ( 25 ) , as demonstrated in Fig . 1C . A short segment of extracellularly recorded sponta- neous cortical ...
... axons project away from cortex , are illustrated in Fig . 1B . The axons of Purkinje cells converge on the deep nucleus neurons ( 25 ) , as demonstrated in Fig . 1C . A short segment of extracellularly recorded sponta- neous cortical ...
Page 66
... axon fragments are observed along with larger clumps of undissociated tissue . The tube is placed in an upright position ... axons . A procedure for the dis- sociation of rabbit dorsal root ganglia which includes Percoll gradient separa ...
... axon fragments are observed along with larger clumps of undissociated tissue . The tube is placed in an upright position ... axons . A procedure for the dis- sociation of rabbit dorsal root ganglia which includes Percoll gradient separa ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml