In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 128
... beaker . The cell suspension is pushed with the plunger through the covered bottom into the beaker to remove chunks of tissue from the freed cells . The filter or mesh should not be allowed to touch the cell suspension once it has been ...
... beaker . The cell suspension is pushed with the plunger through the covered bottom into the beaker to remove chunks of tissue from the freed cells . The filter or mesh should not be allowed to touch the cell suspension once it has been ...
Page 265
... beaker . The perfusion tubing is also placed between the funnel and the beaker such that the medium is able to circulate without disruption . For the biopsy perfusion method , a perfusion tower is used which consists of a 4 - inch glass ...
... beaker . The perfusion tubing is also placed between the funnel and the beaker such that the medium is able to circulate without disruption . For the biopsy perfusion method , a perfusion tower is used which consists of a 4 - inch glass ...
Page 435
... beaker half - filled with 70 % alcohol . 5. Remove sterile gauze from package and place over large beaker . Secure gauze with rubber band . The beaker will be used to contain discarded liquid . We use a 1 - liter Tri - Pour beaker ...
... beaker half - filled with 70 % alcohol . 5. Remove sterile gauze from package and place over large beaker . Secure gauze with rubber band . The beaker will be used to contain discarded liquid . We use a 1 - liter Tri - Pour beaker ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml