In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 139
... Bovine serum albumin ; BPE , bovine pituitary extract ; CT , cholera toxin . Attachment was determined 24 hr after seeding ; CFE and cell number / colony were determined after 7 days . " From T. E. Gray , J. E. Rundhaug , and P ...
... Bovine serum albumin ; BPE , bovine pituitary extract ; CT , cholera toxin . Attachment was determined 24 hr after seeding ; CFE and cell number / colony were determined after 7 days . " From T. E. Gray , J. E. Rundhaug , and P ...
Page 273
... serum under a humidified atmosphere composed of 95 % air / 5 % CO2 ( 2.5 × 10 ° cells / 4 ml / 60 - mm dish ; 0.8 ... bovine serum albumin and 20 % fetal calf serum , and then an equal volume of the same medium containing 0.2 % bovine ...
... serum under a humidified atmosphere composed of 95 % air / 5 % CO2 ( 2.5 × 10 ° cells / 4 ml / 60 - mm dish ; 0.8 ... bovine serum albumin and 20 % fetal calf serum , and then an equal volume of the same medium containing 0.2 % bovine ...
Page 534
... Bovine retinal pigment epithelium culture of , 107 isolation of , 107 Bovine serum , fetal , preparation of , for reaggregate cultures , 30 Bovine serum albumin effects on cell attachment and colony- forming efficiency , 139–140 effects ...
... Bovine retinal pigment epithelium culture of , 107 isolation of , 107 Bovine serum , fetal , preparation of , for reaggregate cultures , 30 Bovine serum albumin effects on cell attachment and colony- forming efficiency , 139–140 effects ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml