In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 77
... Calcium - dependent CAa secretion Induces calcium - dependent CA secretion Induces calcium - dependent CA secretion , increases sodium uptake , inhibits nicotine - induced CA secretion Increases sodium uptake , induces calcium ...
... Calcium - dependent CAa secretion Induces calcium - dependent CA secretion Induces calcium - dependent CA secretion , increases sodium uptake , inhibits nicotine - induced CA secretion Increases sodium uptake , induces calcium ...
Page 92
... calcium may initiate neuronal in- jury leading to death . PC - 12 cells have served as a model to study the effects of neurotoxic agents on cytosolic calcium , and correlations have been made with these actions to neurotoxic events ...
... calcium may initiate neuronal in- jury leading to death . PC - 12 cells have served as a model to study the effects of neurotoxic agents on cytosolic calcium , and correlations have been made with these actions to neurotoxic events ...
Page 179
... calcium release ( 21 ) . Another method which allows sequential characterization of selective effects on both specific receptor - mediated calcium entry as well as calcium release is frequently preferred , however ( 21,31,32 ) . The ...
... calcium release ( 21 ) . Another method which allows sequential characterization of selective effects on both specific receptor - mediated calcium entry as well as calcium release is frequently preferred , however ( 21,31,32 ) . The ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml