In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 49
... centrifuge tube and gas with 5 % CO2 / 95 % O2 until the color turns pale pink - orange ( pH 7.4 ) . Add 1.5 ml of 0.25 % trypsin to the tube of MS , divide the 4 ml of solution equally into two 15 - ml centrifuge tubes , and place them ...
... centrifuge tube and gas with 5 % CO2 / 95 % O2 until the color turns pale pink - orange ( pH 7.4 ) . Add 1.5 ml of 0.25 % trypsin to the tube of MS , divide the 4 ml of solution equally into two 15 - ml centrifuge tubes , and place them ...
Page 120
... Centrifuge at 1000 g ( 2500 rpm ) using a refrigerated swinging - bucket rotor for 20 min . 22. After centrifugation ... centrifuge tube and fill with sterile Joklik's medium . Centrifuge for 10 min at 400 g ( 1400 rpm ) . 24. Combine ...
... Centrifuge at 1000 g ( 2500 rpm ) using a refrigerated swinging - bucket rotor for 20 min . 22. After centrifugation ... centrifuge tube and fill with sterile Joklik's medium . Centrifuge for 10 min at 400 g ( 1400 rpm ) . 24. Combine ...
Page 326
... ( centrifuge door open , centrifuge still off ) . Spin rotor by hand to dislodge air bubbles while pinching tubing on outflow line briefly . Collect water from outflow in a discard beaker . Turn off pump . 5. To start centrifuge , close ...
... ( centrifuge door open , centrifuge still off ) . Spin rotor by hand to dislodge air bubbles while pinching tubing on outflow line briefly . Collect water from outflow in a discard beaker . Turn off pump . 5. To start centrifuge , close ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml