In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 12
... changes . The coverslips , still in racks , are then dipped 10 times each in two changes of fresh deionized water , oven dried , cooled , and transferred from the Chen racks to clean 15 × 100 mm petri dishes for subsequent sterilization ...
... changes . The coverslips , still in racks , are then dipped 10 times each in two changes of fresh deionized water , oven dried , cooled , and transferred from the Chen racks to clean 15 × 100 mm petri dishes for subsequent sterilization ...
Page 13
... changes of boil- ing deionized water without soap and boiled for 10 min in each change . After air drying , the slides are removed from the rack , layered in a Nalgene basket , and soaked overnight in concentrated nitric acid . The ...
... changes of boil- ing deionized water without soap and boiled for 10 min in each change . After air drying , the slides are removed from the rack , layered in a Nalgene basket , and soaked overnight in concentrated nitric acid . The ...
Page 226
... Changes in any of the above parameters over time indicate changes in the viabil- ity or functional competence of the cultured slices . If these changes resulted from the presence of a toxicant , they are taken as indicators of toxicity ...
... Changes in any of the above parameters over time indicate changes in the viabil- ity or functional competence of the cultured slices . If these changes resulted from the presence of a toxicant , they are taken as indicators of toxicity ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml