In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 64
... Coating Each coverslip is coated with polyornithine by applying a small drop ( 25 μl ) of the stock solution . The drop is allowed to stand for 1 hr , and the fluid is then removed by suction . The coverslip is held down with forceps ...
... Coating Each coverslip is coated with polyornithine by applying a small drop ( 25 μl ) of the stock solution . The drop is allowed to stand for 1 hr , and the fluid is then removed by suction . The coverslip is held down with forceps ...
Page 308
... coated with type I collagen than un- coated plastic , but they still do not survive for extended periods in primary cul- ture . Moreover , their porosity ( i.e. , total fenestral surface area ) decreases . In contrast , when cells are ...
... coated with type I collagen than un- coated plastic , but they still do not survive for extended periods in primary cul- ture . Moreover , their porosity ( i.e. , total fenestral surface area ) decreases . In contrast , when cells are ...
Page 378
... Coated with Extracellular Matrix Proteins As mentioned above , if the proximal tubule cells to be cultured are in the form of large tubule fragments , they will attach directly to plastic surfaces . However , when small clusters and ...
... Coated with Extracellular Matrix Proteins As mentioned above , if the proximal tubule cells to be cultured are in the form of large tubule fragments , they will attach directly to plastic surfaces . However , when small clusters and ...
Other editions - View all
In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml