In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 118
... Collagen solution : Prepare a stock solution by diluting 1 part of cold Ethicon ( Somerville , NJ ) and collagen dispersion into 6 parts of cold 50 % methanol and mixing well ( 1 ml collagen ; 3 ml methanol 118 III . Respiratory System.
... Collagen solution : Prepare a stock solution by diluting 1 part of cold Ethicon ( Somerville , NJ ) and collagen dispersion into 6 parts of cold 50 % methanol and mixing well ( 1 ml collagen ; 3 ml methanol 118 III . Respiratory System.
Page 218
... collagen synthesis was an essen- tial requirement for the prolonged culture of tissues containing both epithelial and mesenchymal components . Ascorbic acid and ferrous ions are required for collagen biosynthesis ( 11,12 ) ; ascorbic ...
... collagen synthesis was an essen- tial requirement for the prolonged culture of tissues containing both epithelial and mesenchymal components . Ascorbic acid and ferrous ions are required for collagen biosynthesis ( 11,12 ) ; ascorbic ...
Page 378
... collagen type I which can be purchased in sterilized vials either in solution or as a lyophilized powder . A stock solution of collagen ( 1–3 mg / ml ) in 100 mM acetic acid can be main- tained for long periods of time at 4 ° C ...
... collagen type I which can be purchased in sterilized vials either in solution or as a lyophilized powder . A stock solution of collagen ( 1–3 mg / ml ) in 100 mM acetic acid can be main- tained for long periods of time at 4 ° C ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml