In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 40
... Concentration - Effect Relationships Reaggregates are treated with a range of drug concentrations . This approach is ... concentration - effect relationship for an agent that depletes monoamines , we typically redistribute the ...
... Concentration - Effect Relationships Reaggregates are treated with a range of drug concentrations . This approach is ... concentration - effect relationship for an agent that depletes monoamines , we typically redistribute the ...
Page 170
... concentration - response relationships to a given agonist . As the concentration of the agonist is increased cumulatively ( from left to right ) incremental in- creases in developed force ( y axis ) are noted . Consecutive concentrations ...
... concentration - response relationships to a given agonist . As the concentration of the agonist is increased cumulatively ( from left to right ) incremental in- creases in developed force ( y axis ) are noted . Consecutive concentrations ...
Page 178
... Concentration - Response Relationships After the initial equilibration period , concentration - response relationships can be constructed for selected agonists in the absence or presence of a toxin . These relationships are obtained by ...
... Concentration - Response Relationships After the initial equilibration period , concentration - response relationships can be constructed for selected agonists in the absence or presence of a toxin . These relationships are obtained by ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml